Abstract— The use of pesticide is increasing day by day and it causes a serious concern to the human health, especially some pesticides causes’ deleterious effect causing Cancer. Genotoxicity test has a special significance because it is one of the tests for cancer research and risk assessment. The sprayer has more risk because they directly cause in contact with pesticides. Pesticides may affect the DNA of the body cells of the sprayer and known to cause DNA damage. In this case control study DNA damage due to exposure of pesticides by the Agricultural workers of Jonia area of Barpeta district were assessed using the Comet assay. The result showed that the exposed workers had greater mean comet tail length than those of control i.e. 17.69±5.25 versus 10.72±7.89 (p=0.0034, t=3.8206). Among control group, smokers of exposed group have greater tail length than smokers of control group. When DNA damage in the form of comet tail length and frequency of blood leucocytes showing migration were further evaluated in study group. It was observed that although it is more in elders, vegetarian, alcoholics and smokers but it was it was found significant only in smokers than non smokers. Therefore the present studies suggest that exposure of pesticides and smoking habits may cause DNA damage.
Keywords: Agricultural workers, Pesticide, DNA damage.
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