Abstract— We performed analysis of the biological properties of African swine fever virus (ASF) isolate Odintsovo 02/14. Domestic pigs were inoculated with 50 (low) or 5000 (high) hemadsorbing doses (HAD) of the virus via intranasal (IN) or intramuscular (IM) routes, to investigate the pathogenesis of ASF virus Odintsovo 02/14 isolate. Our results indicated that filtered 10% spleen suspension of ASFV isolate Odintsovo 02/14 induced an acute disease in pigs, resulting in 100% mortality rate. For cultural viral suspension (3rd passage), produced in a PBM cells mortality rate was 85.7%.
We also present an analysis of the complete genome of African swine fever virus (ASF) Odintsovo 02/14 isolate. It is 189 333 nucleotide long and contains more than 160 open reading frames (ORFs). Complete nucleotide sequence of the genome of Odintsovo 02/14 isolate was obtained using pyrosequencing method and used to determine differences between the nucleotide sequences in the genomes of Odintsovo 02/14 and Georgia 01/2007. The genome of ASF virus Odintsovo 02/14 contains substitutions, insertions and deletions in genes encoding structural, membrane, and regulatory proteins, DNA reparation enzymes, host immune response evasion proteins, and MGF genes.
The intergenic region I73R/I329L of Odintsovo 02/14 isolate contains 10-nucleotide long tandem repeat sequence, missing in Georgia 01/2007.
Keywords— African swine fever (ASF), experimental challenge, complete genome sequencing, intergenic region, tandem repeats.
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